Porcine Parvovirus (PPV) Antibody ELISA test kit

Porcine Parvovirus Ab ELISA kit 1.Sensitivity:qualitative analysis 2.Packing : 192 wells/kit 3.Sample:serum 4.ISO9001:2008

Porcine Parvovirus Antibody ELISA t test Kit

1. UsageThe Porcine Parvovirus Antibody ELISA Test kit is used for detection of Porcine Parvovirus Antibody in the serum; assessment of immunity conditions against porcine circovirus in the pig farm and investigation of the epidemiology of the porcine circovirus.2. PrincipleThe kit is based on an indirect enzymatic immunoassay(Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other component s. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer(630 nm).

3.The kit components1 PPV coated Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2pieces (192 wells)2 Negative control serum (green lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 tube (1 mL)3 Positive control serum (red lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 tube (1 mL)4 Anti-pig IgG-HRP conjugate(yellow lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1 bottle (22 mL)5 20 × concentrated washing buffer (white lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .        1 bottle (30 mL)6 Substrate A (purple lid)/B (yellow lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . (12 mL/each)7 Stop solution (blue lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 bottle (12 mL)8 Sample diluent (transparent lid) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 bottle (50 mL)4.Precautions and warnings for users1. Read the use instructions carefully.2. Bring all reagents to room temperature prior to use.3. Do not use components after expiration dates and do not mix components from different lots.4. If the dry bag is red, don ’ t use.5. Don ’ t pollute any components.6. Reseal the plates after use and avoid to wet.7. Do not pipette by mouth

8. Use gloves and Be careful with reagents9. Clean the discarded reagents

5. Test Procedure

1. Take out the coated plates(Can be detached) and record the sample position on a worksheet.Dilute the negative and positive control serum at 1:3. Set 2 wells for negative control serum and 2 wells for positive control serum, 100 μ L/well. S et one blank control well, add 100 μL sample diluent. Similarly, dilute the sample s at 1:40 with the sample diluent,100 μLeach well.Mix gently, incubate at 37 °C for 30 min.2. Wash each well with 300 μ L washing buffer 3 times, and each static for 3 min. The last time,pat to dry on absorbent paper.3 . Add 100 μ L enzyme comjugate into each well, and incubate at 37 °C for 30 min.4 . Repeat step 2.5. Add 50 μL substrate A and 50 μL substrate B into each well, mix properly , incubate for 1 0 min at room temperature in the dark.6. Add stop solution one drop (50 μ L) to each well, and determine the result within 1 0 min.

6.ResultsSet zero for the blank well, and test the OD630 value on the spectrophotometer. For the assay to be valid, the positive control wells' average OD630 value must be greater than or equal to 0.4, and the negative control wells ’ average OD630 value is less than 0.2.If the sample ’ s OD630 value is greater than 0.4, it is judged to be positive; from 0.2 to 0.4, doubtful; and if less than 0.2, negative .

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.

ISO9001:2008 Certificate

Porcine Parvovirus (PPV) Antibody ELISA test kit