Bird Flu elisa kit/Avian Influenza Virus antibody ELISA test kit

Bird Flu antibody ELISA test kit 1.sensitivity:qualitative analysis 2.Spec:96 wells/kit 3.Sample:serum 4.MOQ: 1 kit

Bird Flu/Avian Influenza Virus antibody ELISA test kit

1.Introduction:This kit use AIV solid phase antigen to detect specific antibody against AIV antigen, have high sensitivity, specificity, reproducibility, and is easy to operate quickly and can be used dividually in multiple times .

Reagents  and contents	Coated microtiter strips	concentrated Conjugate solution:	Washing solution:	Substrate A solution	Substrate Bsolution:	Sample dilution:	Stopping solution	Positive contro	Negative control:	Instruction sheet
quantity	8	1	1	1	1	1	1	1	1	1
condition	ready to use	ready to use	ready to use	ready to use	ready to use	ready to use	ready to use	ready to use	ready to use

2. ELISA procedures procedures

1. Diluting concentrated washing solutionDilute the concentrated washing solution with distilled water or deionized water at 1:10, e.g. add 270 ml distilled water in 30 ml concentrated washing solution, and mix thoroughly.2. Sample preparation:Dilute the serum sample with sample dilution at 1:100, e.g. 5 μ l serum + 495 μ l sample dilution,mix thoroughly.3. Adding samples and controlsLeave well A1 as reagent blank. Pipette controls and samples as follows:100 μ l of negative control and positive control respectively, and 100 μ l diluted samples each into remaining wells.Incubation at 37 °C for 30 minutes. Discard liquid of the wells and fill all wells with diluted washing solution, incubate for 1 minute and discard. Repeat washing procedure two more times as above.At the end of the washing step, carefully remove remaining fluid by tapping the strips on th e tissue paper prior to the next step.

Bird Flu elisa kit/Avian Influenza Virus antibody ELISA test kit