Clenbuterol ELISA test kit veterinary drug residue assay

Clenbuterol ELISA test kit 1.Usage:Pork/Liver, Serum, Urine, Feed 2.Sensitivity:0.1ppb 3.Spec.:96 wells/kit 4.ISO9001:2008

   Clenbuterol ELISA test kit

1. Technical specifications

	tissue	serum,urine	feed
detection limit	0.1ppb	0.1ppb	10ppb
recovery rate	80±10%	90±10%	80±15%

2.ELISA procedures1. Bring test kit to the room temperature (20 - 25 °C ) for at least 30 min, note that each reagent must be shaken evenly before use; put the required micro-well strips into plate frames. Re-sealed the unused microplate, stored at 2 - 8 °C , not frozen .2. Numbering: number the micro-wells according to samples and standard solution; each sample and standard solution should be performed in duplicate; record their positions .3. Add 20 µ L of the sample or the standard solution into separate duplicate wells , then add enzyme conjugate, 50 µL/well; antibody working solution , 80 µ L/ well, Mix gently by shaking the plate manuall y, seal the microplate with the cover membrane, incubate at 25 °C for 30 min .4. Pour liq ui d out of microwell, flap to dry on absorbent paper; add 250 µ L/well of tri- deionized water , wash for 4-5 times , marinate for 10 s every time, then take out and flap to dry with absorbent paper .5. Coloration: add 50 µ L of substrate A solution and 50 µ L B solution into each well. Mix gently by shaking the plate manuall y , and incubate at 25 °C for 15 min in the dark for coloration .6. Determination: add 50 µ L of the stop solution into each well. Mix gently by shaking the plate manuall y . Set the wavelength of the microplate reader at 450 nm to determine the OD value of every well. (Recommend to read the OD value at the dual-wavelength 450/630 nm within 5min ) .

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Clenbuterol ELISA test kit veterinary drug residue assay